Princeton University is the principal institution in the new Northeast Region Hub, with the University of Delaware and Rutgers University as partner institutions. The Hub includes five initial affiliates: Lehigh University and Temple University in Pennsylvania, New Jersey Institute of Technology (NJIT), Rowan University in New Jersey, and Delaware State University. The Hub will expand by adding new affiliates each year.

The University of Michigan is the principal institution in the new Great Lakes Region Hub, which also includes: Iowa State University, Missouri University of Science & Technology, Purdue University, University of Akron, University of Chicago, University of Illinois Urbana-Champaign, University of Minnesota Twin Cities, University of Toledo, and the University of Wisconsin–Milwaukee.

Led by the University of Southern California Viterbi School of Engineering, this Hub is a partnership with seven of the top research universities across the western United States, with partners University of Colorado Boulder and the University of California-Los Angeles, and affiliates California Institute of Technology, the Colorado School of Mines, the University of New Mexico, the University of Utah and the University of California-Riverside.

NSF I-Corps Hub NY Region

The City University of New York is the principal institution in the new New York Region Hub, with Columbia University and New York University as partner institutions. Hub affiliates include: The Icahn School of Medicine at Mount Sinai, Rockefeller University, Stevens Institute of Technology, SUNY at Stony Brook, UMass Medical School, and SUNY at Albany.